Cao, Y.; Liu, D.; Zhang, W.-B.* Supercharging SpyCatcher toward an Intrinsically Disordered Protein with Stimuli-Responsive Chemical Reactivity. Chem. Commun. 2017, 53, 8830-8833 (Front Cover). [Link]

Abstract
We report a supercharged, intrinsically disordered protein, SpyCatcher(−), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.